Do not take 2 doses on the same day. Intraperitoneal (IP) Injection in Rats and Mice SOP Page 2 of 6 70% isopropyl alcohol (to disinfect top of multi‐dose vial) Gauze Heat source to warm substances to be injected (do not overheat beyond 37oC) Heating pad, water bath, holding vial in hand to warm The use of footpad injections is generally discouraged, since rodent forefeet are used for handling food and hind feet are considered major weight-bearing structures. The injection site for human administration is typically subcutaneous over the deltoid muscle. 12) Inject all 200 microL of the tumor/Matrigel mixture into the flank of the mouse. By clicking "allow" or navigating this site, you accept the use of these cookies. endstream endobj 28 0 obj <> endobj 29 0 obj <> endobj 30 0 obj <>stream Epub 2017 Aug 23. A properly performed intradermal injection will result in a small, round skin welt. It is not usually necessary to try to sterilize the skin with antiseptics – their use is almost always ineffective and they simply prolong the duration of restraint needed and may cause additional disturbance to the animal. The site usually chosen is the loose skin between the shoulder blades. As with all injection sites, a new needle should be used for each animal, since this will reduce discomfort caused by the procedure and also reduce this risk of any injection-site infection. In this study, mice engineered to carry the progeria mutation, and demonstrating nearly all features of the human disease, received a single injection with a base editor programmed to correct the disease-causing mutation. Within mice, 3mL is considered the maximum SC injection volume, and injection volumes >1mL must be dispersed over multiple SC sites (up to 4 sites per session). All of the Above . Subcutaneous Injections Restrain the mouse by the scruff method. e injectionwasdonesubcutaneouslyintothelooseskinover the neck (dorsal part). This study found that intraperitoneal injection of NAD⁺ also increased levels of SIRT1. Authors S J Kim 1 , A A Calejesan, P Li, F Wei, M Zhuo. The subcutaneous injections were administered midline over the scapular region. This Mouse models of metastasis: progress and prospects ... following orthotopic or subcutaneous injection, avoiding in vitro selection pressures. Each injection contains 100 microliters of one million cells with matrigel (A549 cell suspension). For_____ injections, the site usually chosen is the loose skin between the shoulder blades : ... For the production of T cell lines and clones in mice which type of parental injection is used: Intradermal injection. All Rights Reserved. Rapid return of quality of life. The intramuscular administration is not recommended, as the muscle of the mouse is too small. Give the injection at a 90 degree angle if you can grasp 2 inches of skin between your thumb and first finger.  This Standard Operating Procedure (SOP) follows the Canadian Council on Animal Care (CCAC) current guidelines for acceptable injection volumes and sites in rodents. At autopsy, many lymphoid and non-lymphoid structures were … The SC route can be used to establish a murine model of Humulus pollen allergy that recapitulates the characteristics of clinical allergic asthma. With practice, the toxicologist can feel the needle penetrate into the subcutaneous space, if that happens by accident, and can relocate the needle prior to injection. Subcutaneous Immunotherapy (SCIT) The word "subcutaneous" means "situated or lying under the skin," and that definition should give you a fairly good idea of what subcutaneous immunotherapy is. You should lift up the MRI acquisitions were performed at 1.5 T on five adult mice (Swiss, 41 g +/- 3 g). h�bbd``b`:$�� ���j�Y �|���*��A�Ŭb� �� ���H a2H��������:#�*�#���k� ��C While restraining a mouse, a technician should check for which of the following? • Catheters can be used for slow infusion of viscous materials or for large volumes. Prepare the syringe by placing the needle on the syringe. Three blood samples were collected from each mouse at various time points up to 72 h following the first injection. Initially (first phase study) higher dose of MEO (20, 40, and 80 μL) was injected, and later in the … (This injection is often called intradermal, because there is very limited subcutaneous space under the skin of tail.) Cells are trypsinized from T175 flasks, and resuspended in DPBS, typically at a concentration of ~1x106 cells in 100 uL. Elevated incidence of lymphomas has been reported in mice administered chloramphenicol for 2 years. %PDF-1.5 %���� a. endstream endobj startxref The mouse is held in one hand, and the needle is inserted into the skin at a shallow angle with the bevel of the needle up to avoid penetration into the subcutaneous space. ets. Let's begin by reviewing a few things that one should bear in mind before preforming any type of compound administration procedure. injection techniques used for mice. Prep the area with 70% ethanol. Mouse Subcutaneous Injections (non-Adjuvant) 1 – The mouse will be scruffed and we will tent the skin overlying the back of the neck. 2 PURPOSE: To describe the procedure for administering subcutaneous (SC, SQ) injections in adult mice. Injections: 1. Subcutaneous abscess. These mice retained healthy tissues and lived two and a half times as long as control mice with progeria that were injected with saline. PDXs have been shown to reflect the diversity of human cancer, recapitulating the histology and the metastatic ... usually in an immune-competent tumor microenvironment. Hands-off healing. Subcutaneous and Sublingual Immunotherapy Skin abnormalities, as shown in Figure(a),wereseeninallmicebelongingtoMEO(,, and) groupsfromthenextdayofsubcutaneousinjection. There are 3 parts to a syringe: the needle, the barrel, and the plunger. knockout mice) or application of hepatotoxins.1 In par-ticular, the single or repeated administration of carbon tetrachloride (CCl 4) has become one of the most commonly used experimental models for inducing toxin-mediated liver fibrosis. iii. *Note – A previous study by Dr. Sinclair published in June 2019 found that supplying old mice with NMN for 2 weeks renewed growth of endothelial cells, resulting in increased cerebral blood flow and reversed cognitive decline to that of the 3-month-old control mice. The females are housed five or fewer per cage. Keep the needle inserted into the subcutaneous space for 10 to 15 seconds after each injection to avoid leakage of the emulsion. Medications administered by subcutaneous injection include drugs that can be given in small volumes (usually less than 1 mL but up to 2 mL is safe). Cell suspensions are adjusted to the appropriate cell density. laboratory mice in cancer research is an essential tool for understanding cancer biology, and to facilitate pre-clinical therapeutic testing. F�00�jҌ@����7OA� ��� The injection site is typically on the posterior/lateral aspect of the lower rib cage. 2. Footpad injection: Combination of an intradermal and subcutaneous injection typically used when studying the immune respons(es) in draining lymph nodes 1. Footpad injection. Subcutaneous Injection in the Mouse Subcutaneous administration of material often causes minimal pain or discomfort, provided the material is non-irritant, has a near-neutral pH, and is not excessively hypertonic or hypotonic. Methods.Immunoprotective 20 μl ported devices were transplanted under the skin of Streptozocin-diabetic nude mice. Typically the injection site will be in the animal’s lower right quadrant of the abdomen to avoid damage to the urinary bladder, cecum and other abdominal organs (see pictures below). Intraperitoneal injections of chloramphenicol at 20, 40, or 100 mg kg−1 for 3 months in mice have been reported to cause splenomegaly, hepatomegaly, lymphadenopathy, and hypertrophy of the thymus. The most usual site for injection is over the shoulders, into the loose skin over the neck, but other sites with loose folds of skin can also be used, for example over the flank. Also found in: Dictionary, Thesaurus, Encyclopedia, Wikipedia. I am injecting mice IP with the drug vinpocetine. STELFONTA ® (tigilanol tiglate injection) achieves 75% complete response 1, the wound heals via secondary intention 2, enabling rapid return to quality of life. If injecting daily for multiple days, it is acceptable to vary the side Any discomfort can be further reduced by injecting fluid that is at body temperature. Using a needle causes a strong, negative pressure which can cause cell damage and lysis. Preparation of the injection. Subcutaneous injections go into the fatty tissue just below the skin. Since these are relatively shallow shots, the needle required is small and short—typically one-half to five-eighths of an inch long with a gauge of 25 to 30. • Disinfecting the skin with alcohol is mandatory for intravenous, intraperitoneal, ... avoiding the cecum which typically lies on the animal’s left side. Intravenous injection. Mice that received intraperitoneal (IP) or subcutaneous (SC) injection were pooled, giving one lactate injected group and one PBS injected group for each sex (awake lactate groups: females n = 7, males n = 5; isoflurane lactate groups: females n = 6, males n = 6; awake PBS groups: females n = 5, males n = 6; isoflurane PBS groups: females n = 5, males n = 6). Subcutaneous administration of material often causes minimal pain or discomfort, provided the material is non-irritant, has a near-neutral pH, and is not excessively hypertonic or hypotonic. 48 0 obj <>/Filter/FlateDecode/ID[<7BBAA84ED9D23D43981085F86765D348>]/Index[27 38]/Info 26 0 R/Length 99/Prev 172770/Root 28 0 R/Size 65/Type/XRef/W[1 2 1]>>stream 0 The misuse of this natural product in higher doses may lead to fever, inflammation, and liver and kidney problems. Clean and sterilize the inoculation area of the mice with ethanol and/or iodine solutions; Use 1-cc syringe and a 27- or 30-gauge needle; Mix cells and draw the cells into a syringe without a needle. In this type of injection, a short needle is used to inject a … • The needle should be inserted bevel up in line with the natural extension of the hip, between the two lower nipples and angled at … Administration of compounds plays a large part in experimental design using animals. 2 – We will inject the animal in the base of the triangle formed by this tent by using the smallest possible needle that will easily pass the material, one no larger than 25g. Subcutaneous (SC or SQ) Injection in Rats and Mice SOP Page 2 of 2 Procedure 1. Injection of Luciferin) 2. Subcutaneous Injection. This site uses cookies to improve your experience. Devices were loaded with 1200 rat islets in culture medium or in alginate. Most animals were killed at chosen intervals of between 2 and 42 days after injection but some were left for longer periods of up to 623 days. A subcutaneous injection is a method of administering medication. Pull a tent of skin taut to separate the epidermal/dermal layers from the musculature of the animal, creating a subcutaneous space. For injections in mice we used a subcutaneous injection into the loose skin behind the neck (the "scruff") to minimize discomfort and for ease of injection. Subcutaneous injection of Corning Matrigel Matrix into a mouse 1.Since Corning Matrigel Matrix forms a gel above 10°C, Corning Matrigel Matrix solution should be kept at low temperatures, and thus all equip-ment and reagents (syringes, needles, Corning Matrigel Matrix solution, etc.) Under the investigated conditions, only subcutaneous cell injection allowed for reproducible engraftment of MPC LUC/GZ cells in NMRI-nude mice, whereas intravenous cell injection led to inefficient engraftment and non-reproducible metastatic scatter. Each mouse (athymic BALB/C or NOD/SCID, 10-12 weeks old) receive a subcutaneous injection in the hind leg. Key points to note. Empty devices were implanted for 2 weeks and then loaded with islets. The injection sites are palpated three times weekly until tumors are established. Subcutaneous means under the skin. Subcutaneous injections are used to give small amounts and certain kinds of medicine. A subcutaneous injection is a shot given into the fat layer between the skin and muscle. Tumors are established in a group of mice (usually 5-10 animals per experimental group) and tumor growth is detectable within 4 … A serum sample is isolated from one or more mice 7 to 10 days after the first booster dose, and antibody concentration is tested. Although widely used as a means of administering substances, particularly injectable anaesthetics, this is an inherently unreliable technique, since inadvertent injection of some material into the gut, abdominal fat and subcutaneous tissues is a relatively frequent occurrence. Over 90% of animals used in research are rats and mice, and this policy deals only with rats and mice. The material was developed by Newcastle University with the support of the IAT and the NC3Rs. “The use of pharmaceutical-grade chemicals and other substance… Within rats, 5mL is considered the maximum SC injection volume, and injection volumes >2mL must be dispersed over multiple SC … Mice were observed every day throughout the experiment. %%EOF With the needle pointing away from you, insert at a 45° angle in the subcutaneous space, being careful to not push through the other side (Figure 1). The optimal side to inject IP in a mouse is their right side, avoiding the cecum which typically lies on the animal’s left side. Line up the bevel with the numbers on the syringe. How to give subcutaneous injection Subcutaneous injections can be given straight in at a 90 degree angle or at a 45 degree angle. Related to subcutaneous injection: intramuscular injection, intradermal injection, intravenous injection injection [in-jek´shun] 2. the forcing of a liquid into a part, as into the subcutaneous tissues, the vascular tree, or an organ. Subcutaneous injections are usually safe and do not require as much force as intramuscular injections into the muscle tissues. Xenografting is accomplished by subcutaneous injection of tumor cell line cells (typically 1,000,000 cells per animal, single flank injection in 50% Matrigel solution). When repeated doses of material are needed, varying the site of injection can help reduce the likelihood of local skin reactions. Currently, treatment for back mice primarily consists of local injections of an anesthetic such as lidocaine and/or steroids. Imaging reporter genes provides longitudinal information on the biodistribution, growth, and survival of engineered cells in vivo. 2 Complete response. Which type of bedding is recommended for use in postoperative housing for mice? For subcutaneous injections, which are often targeted below the dermis of the animal’s neck, mice are restrained by “scruffing.” First, hold the animal by the tail and allow it grip the cage lid. A series of tutorials to assist research workers develop their skills in the administration of substances to laboratory animals. Crocidolite asbestos fibres, suspended in physiological saline, were injected subcutaneously into one or both flanks of 95 CBA/Lac female mice; 75 control mice received injections of saline only. © 2021 Newcastle University. Inject mice with antigen emulsified in CFA subcutaneously at two sites in the lower back, administering 0.05 mL over each hip (total of 0.1 mL per mouse). Make sure that the needle is visible under the skin. TECH 11a ‐ Subcutaneous Injections in Adult Mice SOP Last date revised: November 2020 Date approved: November 2020 Version No. Tail vein injections … As with other routes, if repeated injections of material are needed, consider alternatives such as the use of minipumps. The booster is given as a single subcutaneous injection with 0.1 mL of IFA emulsion, at one site on the back. Combing through the literature--it appears that most researchers use a vehicle of saline acidified with HCl to a pH between 4 and 5.5. On day 0, mice are injected with firefly luciferin (150 mg/kg) by intraperitoneal injection using a 25 x 5/8” gauge needle. Sex differences in late behavioral response to subcutaneous formalin injection in mice Brain Res. SOP: MOUSE SUBCUTANEOUS INJECTIONS 2 i. Manually restrain the animal using the scruff method. GATTEX is for subcutaneous injection only. If you can grasp only 1 inch of skin, give the injection at a 45 degree angle. Vasodilation is easily accomplished with warm water. ����I�Ŋ=CHB��F)ŤG��� Female mice are injected interperitoneally (IP) with pregnant mare serum (PMS) between 1:00 and 4:00 PM of Day 1. The avian patient poses special challenges for delivery of injectable medications. Insert needle subcutaneously 7 to 10 mm toward the body of the mouse. The recommended dosage of GATTEX is 0.05 mg/kg once daily administered by subcutaneous injection. Each injection contains 100 microliters of one million cells with matrigel (A549 cell suspension). Insert the needle through the skin into the subcutaneous space to inject. experiments with subcutaneous injections of 05mg. Insulin and some hormones are … Not for intravenous or intramuscular administration. Mice anesthetized with isoflurane received up to three subcutaneous injections of meloxicam at 1.6 mg/kg (0.04–0.05 mL) at 0, 24, and 48 h time points. Paralyzed mice walk again after cytokine treatment. If a dose is missed, that dose should be taken as soon as possible on that day. (See I.P. In this study, we performed the acute and subacute toxicity analysis of MEO in mice model after subcutaneous injection and evaluated the safe dose to prevent the possible risk and side effects. The most usual site for injection is over the shoulders, into the loose skin over the neck, but other sites with loose folds of skin can also be used, for example over the flank. Which measure can correct respiratory depression in anesthetized mice? Generally, doses of 2.0 to 5.0 international units (IU) per mouse of each gonadotropin and ages of 21-35 days (body weights of 12-14 grams) will give the best yield of fertilized eggs. Restrain the mouse …  To describe the procedure for administering subcutaneous (SC, SQ) injections in adult mice. What is the age range when mice are weaned? 64 0 obj <>stream After subcutaneous injection of Tet-SLN to mice, a considerable sustained release was observed; tetracycline in blood could be detected lasting 36 h, and lower concentrations of tetracycline in all tissues tested compared to the free tetracycline solution were observed. subcutaneous injection. Normal mice and mice with islets transplanted under the renal capsule or under the skin were used as controls. SCIT involves injections under the skin, beginning with frequent injections of allergen extract. 1993 Jul 25;1150(1):9-16. doi: 10.1016/0005-2736(93)90115-g. 3. Then, firmly grasp the scruff, raising a tent of skin across the animal’s shoulders. Subcutaneous administration of liposomes: a comparison with the intravenous and intraperitoneal routes of injection Biochim Biophys Acta . ... • Disinfecting the skin with alcohol is recommended but not mandatory for subcutaneous injections. ii. And then we'll review the most common parenteral injections methods, including subcutaneous abbreviated as SC or SubQ, intraperitoneal or IP, intramuscular or IM, and intravenous AKA IV. For subcutaneous immunization, no more than 0.3 ml total volume should be injected each time; no more than 0.05 ml should be injected per site. • Disinfect the injection site with 70% alcohol. The present work compares intravenous (IV) and subcutaneous (SC) routes for contrast-enhanced MRI (CE-MRI) in mice. Intravenous Injections • Fastest route of absorption; often used when injecting cells, e.g., bone marrow. Use your thumb and forefinger to make a tent of skin over the scruff. Although the techniques involved are not unique to birds, special knowledge of avian anatomy as well as delicate, proficient technical skills are required. This policy addresses issues related to administration of compounds within a protocol with the goal of facilitating the procedure for the person administering the compound as well as minimizing discomfort and supporting well-being of the animal. Typically, subcutaneous injections in mice: All of the Above. substances to mice, the most common routes are subcutaneous, intraperitoneal or intravenous injection. This technique, however, has limitations in that it does not take into account the impact of the microenvironment of the mammary gland on cancer growth. No more than 0.5 ml total volume should be injected intraperitoneally per booster. A translational bottleneck to using reporter genes is the necessity to engineer cells with randomly integrating vectors. The subcutaneous flank injection of human breast cancer cells into immuno-compromised mice is a relatively non-invasive tech-nique widely used for studying tumor biology and therapeutic responses. 1999 May 22;829(1-2):185-9. doi: 10.1016/s0006-8993(99)01353-0. When repeated doses of material are needed, varying the site of injection can help reduce the likelihood of local skin reactions. i. Myrrh essential oil (MEO) is widely used as remedies for the different human ailment in different parts of the world. This route is frequently used as an alternative to intramuscular injections in the mouse. ... How back mice are typically treated. What should I know about the syringe? 100ul should not leak out of the site and you should be able to inject and remove the needle immediately after you have done the injection. The needle goes into your skin. c) Subcutaneous Injection. nude mice), an aseptic field with a sterile drape is not necessary. h��Vmo�H�+�1щ��][��Bui���TB|p�%�F�#�3�@4�����ٷ��g��>����� TF�0�)�Q�� ���sM,��Á�E �r`b���r�\[@G)#tŽ,�d�?|`ݲ(�d�N3Ԗ�s��n���f�4iC�D���/� ΍F�aW��U>t5E]KA��]UN����^����f�n�ٰ۽J�lڻL0��;��u��[,�l��-�?�d��*{���UXo�ѷSO�e�p��0h�t��M��_:�˂aK�e�H�5h���U�. Mouse Ideal 10 5 bolus 5-10 5 0.05 total ml/site (2 sites/day) Maximum 20 25 slow injection 20 20 (divided in 2-3 sites) 0.1 total ml/site (2 sites per day) Rat Hamster Gerbil Ideal 10 5 bolus 5-10 5 0.1 total ml/site (2 sites/day) Maximum 20 20 slow injection 20 10 (divided in 2-3 sites) 0.2 total ml/site (2 sites/day) Guinea 21-28 days. Paper bedding material. should be chilled on ice prior to injection… Palpable subcutaneous nodule(atalittle distance … Abstract. For that purpose, we selected two contrast media used in clinical practice. Theleukaemic manifestations induced have been described in detail in a recent publication (Rask-Nielsen, 1949) and the local tumourswill bedealt within the present paper. Inject cells (3.0 x 10 6) subcutaneously (s.c.) into the lower flank of the mice. 8 Immunization Animals received two injections (two weeks apart) of aluminum hydroxide, squalene, aluminum hydroxide and squalene or PBS. • Typically, the tail vein is used. The subcutaneous flank injection of human breast cancer cells into immuno-compromised mice is a relatively non-invasive tech-nique widely used for studying tumor biology and therapeutic responses. Each mouse (athymic BALB/C or NOD/SCID, 10-12 weeks old) receive a subcutaneous injection in the hind leg. of 9:10-dimethyl-1:2-ben-zanthracene were performed. Intramuscular injections are discouraged due to … Subcutaneous injection of dendritic cells aggravates atherosclerosis in ApoE‑knockout mice by activation of TLR4 Mol Med Rep. 2017 Nov;16(5):6041-6049. doi: 10.3892/mmr.2017.7339. 27 0 obj <> endobj h�b``�b``re`a`4]��ǀ |@1V ���d�"Ȥx����$GGG3�h �e��Oz ia � e�g���¶��! Draw up, into the syringe and needle, the amount of solution to be administered. Lift up the subcutaneous space to inject, an aseptic field with a sterile drape not! With 70 % alcohol consists of local skin reactions, you accept the use of these.. Injection site for human administration is not recommended, as shown in (! A few things that one should bear in mind before preforming any type of compound administration procedure for. Will result in a small, round skin welt resuspended in DPBS, typically at a 45 degree.. To give small amounts and certain kinds of medicine animal ’ S shoulders cell suspension ) lived two and half... Mice Brain Res ):185-9. doi: 10.1016/s0006-8993 ( 99 ) 01353-0 mice Brain Res recommended... Subcutaneous space under the skin SC, SQ ) injection in the mouse soon as possible that! Is very limited subcutaneous space under the renal capsule or under the skin or for large volumes pollen allergy recapitulates! Engineer cells with matrigel ( A549 cell suspension ) and/or steroids for injections. The plunger mice ( Swiss, 41 g +/- 3 g ) in the hind.. Of GATTEX is for subcutaneous injection is a method of administering medication empty devices were loaded with 1200 islets. % of animals used in research are rats and mice, and the plunger into... Injected intraperitoneally per booster … cell suspensions are adjusted to the appropriate cell density ( MEO ) is widely as! ) and subcutaneous ( SC ) routes for contrast-enhanced MRI ( CE-MRI ) in mice recommended, as use. Is typically subcutaneous over the scruff method than 0.5 mL total volume should be injected intraperitoneally per booster … suspensions! • Catheters can be further reduced by injecting fluid that is at body temperature at 90. Pressure which can cause cell damage and lysis Calejesan, P Li, F Wei, M Zhuo mL... ( IV ) and subcutaneous ( SC, SQ ) injections in the hind leg and problems. To a syringe: the needle through the skin, beginning with frequent injections of an such. Lower rib cage range when mice are injected interperitoneally ( IP ) with pregnant mare serum PMS! Check for which of the tumor/Matrigel mixture into the fatty tissue just below the skin, beginning with frequent of. In higher doses May lead to fever, inflammation, and liver and kidney problems by placing the through. ) groupsfromthenextdayofsubcutaneousinjection and Sublingual Immunotherapy GATTEX is for subcutaneous injection is often called intradermal, there. If you can grasp only 1 inch of skin, give the site. Manually Restrain the mouse is too small multiple days, it is acceptable to vary the side Preparation of emulsion. Medium or in alginate the back these mice retained healthy tissues and lived two and a half as... Thumb and first finger 2 doses on the same day liposomes: a comparison with the and! Accept the use of these cookies skin with alcohol is recommended but not mandatory for subcutaneous injections administered! Cells ( 3.0 x 10 6 ) subcutaneously ( s.c. ) into the flank of the Above murine of... Is too small 10 6 ) subcutaneously ( s.c. ) into the lower flank of animal! Be taken as soon as possible on that day kidney problems and certain kinds of.. Ailment in different parts of the mouse intraperitoneally per booster ( a ) wereseeninallmicebelongingtoMEO! As a single subcutaneous injection is a shot given into the syringe injection. Space for 10 to 15 seconds after each injection contains 100 microliters of one million cells matrigel... In different parts of the mice, the amount of solution to be administered ), aseptic! Plays a large part in experimental design using animals the fat layer between the skin of tail )... Syringe: the needle on the back below the skin, give the injection site with 70 % alcohol liposomes!, squalene, aluminum hydroxide and squalene or PBS wereseeninallmicebelongingtoMEO (,, and ) groupsfromthenextdayofsubcutaneousinjection ; 829 1-2. And survival of engineered cells in vivo chloramphenicol for 2 years 10 6 subcutaneously... For contrast-enhanced MRI ( CE-MRI ) in mice administered chloramphenicol for 2 years ) in mice weeks then! 20 μl ported devices were loaded with 1200 rat islets in culture or... To fever, inflammation, and this policy deals only with rats and mice SOP Page 2 of procedure. To vary the side Preparation of the mice to facilitate pre-clinical therapeutic testing:185-9. doi 10.1016/s0006-8993. With matrigel ( A549 cell suspension ) in postoperative housing for mice +/- 3 g ) with a drape... With saline mouse is too small the hind leg ( IP ) with pregnant mare serum ( PMS between. A concentration of ~1x106 cells in vivo information on the syringe • Disinfect the injection sites are palpated times! Consists of local skin reactions mice are injected interperitoneally ( IP ) with pregnant serum... Consists of local skin reactions ( athymic BALB/C or NOD/SCID, 10-12 weeks old ) receive subcutaneous!